Institut de Neurosciences Cognitives et Intégratives d'Aquitaine (UMR5287)

Aquitaine Institute for Cognitive and Integrative Neuroscience

Université de Bordeaux

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33076 Bordeaux cedex


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CNRS Ecole Pratique des Hautes Etudes Université de Bordeaux

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A Rodent Model of Mild Neonatal Hypoxic Ischemic Encephalopathy

Julien Gotchac, Laura Cardoit, Muriel Thoby-Brisson and Olivier Brissaud

by Loïc Grattier - published on

1. Institut des Neurosciences Cognitives et Intégratives d’Aquitaine, CNRS UMR 5287, University of Bordeaux, Bordeaux, France
2. Pediatric Intensive Care Unit, Teacher’s hospital of Children Pellegrin, Bordeaux, France

Hypoxic Ischemic Encephalopathy (HIE) in neonates results in cerebral injuries with potential dramatic consequences for life. Several animal models of HIE have been developed, but none closely replicate human cases (single carotid ligation protocol). We aimed to develop a novel and more accurate HIE model in juvenile (post-natal days (PND) 14–16) rats. We induced a 9 min hypoxic cardiac arrest (CA) by stopping mechanical ventilation of intubated, ventilated and curarized rats followed by a cardiopulmonary resuscitation. We then performed radiological (cerebral MRI), behavioural (Open Field, Elevated Plus Maze, Fear Conditioning), and histological (Cresyl Violet and Fluoro-Jade B) testing on treated animals. Rats in the CA group developed an anxiolytic-like behavioral profile in adulthood without any locomotor impairment, nor memory deficits. MRI investigation performed early after CA failed to reveal any change in apparent diffusion coefficient (ADC) in brain tissue suggesting no massive anatomical lesion had occurred. In contrast (see figure below), signs of neurodegeneration were found in the Dentate Gyrus and the CA1 region of the hippocampus at day 1 post-CA, suggesting that the anxiolytic-like phenotype observed in adulthood could be related to an abnormal degeneration of this brain region beginning immediately after CA. Our model constitutes a potential model for studying mild, yet persistent and region-specific cerebral injury resulting from an acute oxygen deprivation.

Gotchac J, Cardoit L, Thoby-Brisson M and Brissaud O. (2021) A Rodent Model of Mild Neonatal Hypoxic Ischemic Encephalopathy. Front. Neurol. 12:637947. doi: 10.3389/fneur.2021.637947

View online : A Rodent Model of Mild Neonatal Hypoxic Ischemic Encephalopathy Julien Gotchac1*, Laura Cardoit1, Muriel Thoby-Brisson1† and Olivier Brissaud1,2*†